Sponges are at a cellular level of organization and thus do not have organs or even well-developed tissues; nevertheless, they produce sperm and eggs and also reproduce asexually. Some species of sponge are monoecious, others are dioecious. Sperm and eggs are formed by aggregations of cells called amoebocytes in the body wall; these are not considered gonads because of their origin and transitory nature.

In hydrozoan coelenterates, temporary gonads are formed by groups of cells in either the epidermis (outer cell layer) or gastrodermis (gut lining), depending on the species; scyphozoan and anthozoan coelenterates generally have gonads in the gastrodermis. The origin and development of gonads in coelenterates, particularly freshwater species, are often associated with the seasons. Freshwater hydrozoans, for example, reproduce asexually until the onset of cold weather, which stimulates them to form testes and ovaries. Colonial hydrozoans asexually produce individuals known as polyps. Polyps, in turn, give rise to free swimming stages (medusae), in which gonads develop. The body organization of sponges and coelenterates is such that most of their cells are in intimate contact with the environment; consequently, gametes are shed into the water, and no ducts are necessary to convey them to the outside.

In contrast to sponges and coelenterates, platyhelminths generally have well-developed organ systems of a permanent nature and, in addition, have evolved secondary reproductive structures to convey sex products. One exception is the acoels, a group of primitive turbellarians; they lack permanent gonads, and germinal cells develop from amoebocytes in much the same manner as in sponges. The majority of flatworms, however, are monoecious, the primary sex organs consisting of one or more ovaries and testes. The tube from the ovary to the outside is called the oviduct; it often has an outpocketing (seminal receptacle) for the storage of sperm received during copulation. In many species the oviduct receives a duct from yolk (vitelline) glands, whose cells nourish the fertilized egg. Beyond the entrance of the duct from the yolk glands the oviduct may be modified to secrete a protective capsule around the egg before it is discharged to the outside. The male organs consist of testes, from which extend numerous tubules (vasa efferentia) that unite to form a sperm duct (vas deferens); the latter becomes an ejaculatory duct through which sperm are released to the outside. The sperm duct may exhibit expanded areas that store sperm (seminal vesicles), and it may be surrounded by prostatic cells that contribute to the seminal fluid. The sperm duct eventually passes through a copulatory organ. The same basic structural pattern, somewhat modified, is found in most higher invertebrates.

Aschelminthes (roundworms) are mostly dioecious; frequently there are external differences between males and females (sexual dimorphism). The males are generally smaller and often have copulatory spicules. Nematodes have relatively simple reproductive organs, a tubular testis or ovary being located at the end of a twisted tube. The portion of the female tract nearest the ovary forms a uterus for temporary storage of fertilized eggs. Some species lay eggs, but others retain the egg in the uterus until the larva hatches. The sperm are released into a cavity called the cloaca. A number of free-living nematodes are capable of sex reversal—if the sex ratio in a given population is not optimal or if environmental conditions are not ideal, the ratio of males to females can be altered. This sometimes results in intersexes; i.e., females with some male characteristics. Hermaphroditism occurs in nematodes, and self-fertilization in such species is common. Unisexual reproduction among rotifers is described below (see Parthenogenesis).