Contribution of other sciences

Appreciation of the cell as the unit of life has accrued from important sources other than microscopy; perhaps the most important is microbiology. Even though the small size of microorganisms prohibited much observation of their detailed structure until the advent of electron microscopy, they could be grown easily and rapidly. Thus it was that French chemist and microbiologist Louis Pasteur’s studies of microbes published in 1861 helped to establish the principle of biogenesis—namely, that organisms arise only by the reproduction of other organisms. Fundamental ideas regarding the metabolic attributes of cells—that is, their ability to transform simple nutritional substances into cell substance and utilizable energy—came from microbiology. Pasteur perhaps overplayed the relation between catalysis and the living state of cells in considering enzymatic action to be an attribute of the living cell rather than of the catalytic molecules (enzymes) contained in the cell; it is a fact, however, that much of cell chemistry is enzyme chemistry—and that enzymes are one defining attribute of cells. The techniques of microbiology eventually opened the way for microbial genetics, which in turn provided the means for solving the fundamental problems of molecular biology that were inaccessible at first to direct attack by biochemical methods.

The science of molecular biology would be most capable of overthrowing the cell theory if the latter were an exaggerated generalization. On the contrary, molecular biology has become the foundation of cell science, for it has demonstrated not only that basic processes such as the genetic code and protein synthesis are similar in all living systems but also that they are made possible by the same cell components—e.g., chromosomes, ribosomes, and membranes.

In the overlapping histories of cell biology and medicine, two events are especially important. One, the identification in 1827 by Prussian-Estonian embryologist Karl Ernst Ritter von Baer of the ovum (unfertilized egg) as a cell, was important considering the many ways it often differs from other cells. Baer not only laid the foundations for reproductive biology but also provided important evidence for the cell theory at a critical time. The second important event was the promotion in 1855 of the concept of “cellular pathology” by Virchow. His idea that human diseases are diseases of cells and can be identified and understood as such gave an authority to cell theory.

Although biochemistry might have made considerable progress without cell theory, each influenced the other almost from the start. When it was established that most biochemical phenomena are shared by all cells, the cell could be defined by its metabolism as well as by its structure. Cytochemistry, or histochemistry, made a brilliant start in 1869, when Swiss biochemist Johann Friedrich Miescher postulated that the nucleus must have a characteristic chemistry and then went on to discover nucleic acids, which have since been shown to be the crucial molecules of inheritance and metabolism.

Cell theory by itself cannot explain the development and unity of the multicellular organism. A cell is not necessarily an independently functioning unit, and a plant or an animal is not merely an accumulation of individual cells. Fortunately, however, the long controversy centring upon the individuality and separateness of cells has ended. Cell biology now focuses on the interactions and communication among cells as well as on the analysis of the single cell. The influence of the environment on the cell has always been considered important; now it has been recognized that one important part of the environment of a cell is other cells.

Cell theory thus is not so comprehensive as to eliminate the concept of the organism as more than the sum of its parts. But the study of a particular organism requires the investigation of cells both as individuals and as groups. The problem of cancer is an example: a plant or animal governs the division of its own cells; the right cells must divide, be differentiated, and then be integrated into the proper organ system at the right time and place. Breakdown results in a variety of abnormalities, one of which is cancer. When the cell biologist studies the problem of the regulation of cell division, the ultimate objective is to understand the effect of the whole organism on an individual cell.