- On the Web:
- Harvard Health Publishing - Does metabolism matter in weight loss? (Dec. 21, 2024)
Food materials must undergo oxidation in order to yield biologically useful energy. Oxidation does not necessarily involve oxygen, although it must involve the transfer of electrons from a donor molecule to a suitable acceptor molecule; the donor is thus oxidized and the recipient reduced. Many microorganisms either must live in the absence of oxygen (i.e., are obligate anaerobes) or can live in its presence or its absence (i.e., are facultative anaerobes).
If no oxygen is available, the catabolism of food materials is effected via fermentations, in which the final acceptor of the electrons removed from the nutrient is some organic molecule, usually generated during the fermentation process. There is no net oxidation of the food molecule in this type of catabolism; that is, the overall oxidation state of the fermentation products is the same as that of the starting material.
Organisms that can use oxygen as a final electron acceptor also use many of the steps in the fermentation pathways in which food molecules are broken down to smaller fragments; these fragments, instead of serving as electron acceptors, are fed into the TCA cycle, the pathway of terminal respiration.
In this cycle all of the hydrogen atoms (H) or electrons (e−) are removed from the fragments and are channeled through a series of electron carriers, ultimately to react with oxygen (O; see below Energy conservation). All carbon atoms are eliminated as carbon dioxide (CO2) in this process. The sequence of reactions involved in the catabolism of food materials may thus be conveniently considered in terms of an initial fragmentation (fermentation), followed by a combustion (respiration) process.
The catabolism of glucose
Glycolysis
Quantitatively, the most important source of energy for cellular processes is the six-carbon sugar glucose (C6H12O6). Glucose is made available to animals through the hydrolysis of polysaccharides, such as glycogen and starch, the process being catalyzed by digestive enzymes. In animals, the sugar thus set free passes from the gut into the bloodstream and from there into the cells of the liver and other tissues. In microorganisms, of course, no such specialized tissues are involved.
The fermentative phase of glucose catabolism (glycolysis) involves several enzymes; the action of each is summarized below. In living cells, many of the compounds that take part in metabolism exist as negatively charged moieties, or anions, and are named as such in most of this article (e.g., pyruvate, oxaloacetate).
In order to obtain a net yield of ATP from the catabolism of glucose, it is first necessary to invest ATP. During step [1] the alcohol group at position 6 of the glucose molecule readily reacts with the terminal phosphate group of ATP, forming glucose 6-phosphate and ADP. For convenience, the phosphoryl group (PO32−) is represented by Ⓟ. Because the decrease in free energy is so large, this reaction is virtually irreversible under physiological conditions.
In animals, this phosphorylation of glucose, which yields glucose 6-phosphate, is catalyzed by two different enzymes. In most cells a hexokinase with a high affinity for glucose—i.e., only small amounts of glucose are necessary for enzymatic activity—effects the reaction. In addition, the liver contains a glucokinase, which requires a much greater concentration of glucose before it reacts. Glucokinase functions only in emergencies, when the concentration of glucose in the blood rises to abnormally high levels.
Certain facultative anaerobic bacteria also contain hexokinases but apparently do not use them to phosphorylate glucose. In such cells, external glucose can be utilized only if it is first phosphorylated to glucose 6-phosphate via a system linked to the cell membrane that involves a compound called phosphoenolpyruvate (formed in step [9] of glycolysis), which serves as an obligatory donor of the phosphate group; i.e., ATP cannot serve as the phosphate donor in the reaction.
The reaction in which glucose 6-phosphate is changed to fructose 6-phosphate is catalyzed by phosphoglucoisomerase [2]. In the reaction, a secondary alcohol group (―C∣HOH) at the second carbon atom is oxidized to a keto-group (i.e., ―C∣=O), and the aldehyde group (―CHO) at the first carbon atom is reduced to a primary alcohol group (―CH2OH). Reaction [2] is readily reversible, as is indicated by the double arrows.
The formation of the alcohol group at the first carbon atom permits the repetition of the reaction effected in step [1]; that is, a second molecule of ATP is invested. The product is fructose 1,6-diphosphate [3]. Again, as in the hexokinase reaction, the decrease in free energy of the reaction, which is catalyzed by phosphofructokinase, is sufficiently large to make this reaction virtually irreversible under physiological conditions; ADP is also a product.
The first three steps of glycolysis have thus transformed an asymmetrical sugar molecule, glucose, into a symmetrical form, fructose 1,6-diphosphate, containing a phosphoryl group at each end; the molecule next is split into two smaller fragments that are interconvertible. This elegant simplification is achieved via steps [4] and [5], which are described below.
The aldolase reaction
In [4], an enzyme catalyzes the breaking apart of the six-carbon sugar fructose 1,6-diphosphate into two three-carbon fragments. The molecule is split between carbons 3 and 4. Reversal of this cleavage—i.e., the formation of a six-carbon compound from two three-carbon compounds—is possible. Because the reverse reaction is an aldol condensation—i.e., an aldehyde (glyceraldehyde 3-phosphate) combines with a ketone (dihydroxyacetone phosphate)—the enzyme is commonly called aldolase. The two three-carbon fragments produced in step [4], dihydroxyacetone phosphate and glyceraldehyde 3-phosphate, are also called triose phosphates. They are readily converted to each other by a process [5] analogous to that in step [2]. The enzyme that catalyzes the interconversion [5] is triose phosphate isomerase, an enzyme different from that catalyzing step [2].